FIG. 1.

Competitive binding of immobilized fimbriae to their antibody as determined by ELISA. Fimbriae that were applied to the surface of the titer plate wells competed with suspended fimbrial beads for rabbit anti-K88ac fimbrial antibodies. Values show percent inhibition obtained with fimbriae covalently bound to Sepharose 4B (▾) and fimbriae prior to being bound to Sepharose 4B (●). BSA was not bound to the Sepharose 4B-fimbrial column (data not shown). The initial concentration of the competitor was 10 μg/100 μl. See Materials and Methods for further details.