FIG. 2.

Affinity purification of fimbrial binding receptor from intestinal mucus by using a column containing fimbriae covalently bound to Sepharose 4B-fimbrial beads. A mucus extract (30 ml containing 60 mg of protein) was applied to the column, and the column was washed successively with 30 ml each of buffers A, B, and C (arrows). The absorbency of the eluate at 280 nm was approximately 0.001 OD unit when buffer C (the receptor elution buffer) was applied. Application of 30 ml of BSA in buffer A followed by washing and elution with buffers A, B, and C yielded a pattern similar to that obtained with mucus, except no BSA was eluted with buffer C. The total protein in the peak eluted after 20 ml was approximately 600 μg. See Materials and Methods for further details.