TABLE 1.

Effect of incubation time and buffer composition for blood collection on C. albicans ATCC 66369 and C. glabrata platelet fixation^a

Candida spp.	Blood collection buffer	% of yeast cells bound to platelets in blood from:
		Inoculated mice at:			Uninfected miceb at:
		<1 min	5 min	30 min	<1 min	5 min	30 min
C. albicans	PBS	92.0 ± 0.6	90.0 ± 0.4	88.0 ± 0.4	3.0 ± 0.7	5.0 ± 0.8	47.4 ± 0.8
	PBS-For	96.2 ± 0.3	95.0 ± 0.2	87.0 ± 1.8	1.2 ± 0.3	1.8 ± 0.8	0.8 ± 0.2
C. glabrata	PBS	98.0 ± 0.3	98.2 ± 0.6	93.0 ± 0.4	0.2 ± 0.1	0.5 ± 0.1	66.2 ± 0.9
	PBS-For	98.7 ± 0.8	97.5 ± 0.6	95.8 ± 0.4	0.2 ± 0.1	0.4 ± 0.2	0.2 ± 0.1

^aMice were inoculated as described in Materials and Methods, and blood recovered by periorbital puncture was incubated for 1, 5, and 15 min in 0.15 M PBS (pH 7.2) or PBS–3.3 mM formaldehyde (PBS-For) prior to examination. Data are means ± standard deviations of three observations of six independent experiments. 

^bNegative controls were performed by incubating blood from uninfected mice under the same conditions as those for blood from infected mice and with the same buffers supplemented with Candida cells.