FIG. 2.
Neutrophil and mononuclear cell populations of mouse spleens during systemic infection by M. tuberculosis. Mice were injected i.v. with 105 CFU of M. tuberculosis and treated i.v. with the neutrophil-depleting MAb RB6-8C5 or with PBS (control). The antibody was administrated at 5 h, 2 days, and 4 days of infection. At the indicated time points, groups of four or five mice were sacrificed and spleen cell suspensions were obtained. After erythrocyte lysis, cells were washed and total and differential leukocyte counts were performed. Bars represent means and standard deviations. ND, not detectable. Statistical differences between noninfected and infected mice: ∗, P < 0.05; ∗∗, P < 0.01. Results are from one representative experiment of two independent experiments.