FIG. 4.
Semiquantitative RT-PCR analysis of in vivo expression of IFN-γ, iNOS, and HPRT in mouse livers at 3 and 7 days after systemic infection with 105 CFU of M. tuberculosis. Liver fragments were collected from control mice (mice injected with PBS) and from mice made neutropenic by treatment with MAb RB6-8C5 as described for Fig. 2. Each lane represents data from one animal after scanning of the Southern blots. Results are from one representative experiment of two independent experiments.