Table 3.
Assessment of intra and inter plate coefficient of variation (CV) using synovial fluid, vendor plasma quality control (QC) and serum QC samples. Only data from biomarkers that had values above lower limit detection are presented.
| Synovial fluid | Vendor plasma QC | ||
|---|---|---|---|
| Intra plate | Biomarkers | ||
| Total, n | 63–72 | 71–73 | |
| Intra plate CV %, mean (range) | 6.3 (0.0–25.0) | 6.2 (0.0–30.1) | |
| With CV < 20%, n (%) | 61–72 (97–100) | 59–72 (80–100) | |
| aExperiments, n | 2 | 3 | |
| Samples, n (dilution) | 4 (4x) | 1 (1x) | |
| Repeats/plate, n | 2 or 4 | 2 | |
| Plates, n | 2 | 32 | |
| Synovial fluid | Vendor plasma QC | Serum QC | ||
|---|---|---|---|---|
| Inter plate | Biomarkers | |||
| Total, n | 66 | 67–73 | 78 | |
| Inter plate CV %, mean (range) | 19.1 (6.7–49.6) | 13.5 (0.4–79.3) | 14.8 (5.3–92.5) | |
| With CV < 20%, n (%) | 42 (64) | 59–70 (81–99) | 68 (87) | |
| Experiments, n | 1 | 3 | 1 | |
| Samples, n (dilution) | 1 (4x) | 1 (1x) | 1 (1x) | |
| Repeats/plate, n | 1 | 2 | 1 | |
| Plates, n | 9–12 | 34 | 15–19 | |
a
Experiments = analyses done at one, two or three different occasions at Olink.