Table 4.
Assessment of inter experimental coefficient of variation (CV) using synovial fluid, serum and plasma quality control (QC) samples. The data were either adjusted (using 14 bridging samples) or unadjusted between two experiments. Only data from biomarkers that had values above lower limit of detection are presented.
| Synovial fluid |
Serum |
Vendor plasma QC |
|||
|---|---|---|---|---|---|
| No adjustment | No adjustment | Adjusted | No adjustment | Adjusted | |
| Biomarkers | |||||
| Total n | 63 | 70 | 70 | 64 | 64 |
| Inter experiment CV %, mean (range) | 33.0 (0.1–114.6) | 30.3 (0.0–134.0) | 17.7 (0.0–126.3) | 24.8 (0.1–130.3) | 9.0 (0.1–75.5) |
| With CV < 20%, n (%) | 16 (25) | 23 (33) | 57 (81) | Nd | 60 (95) |
| aExperiments, n | 2 | 2 | 2 | 2 | 2 |
| Samples, n (dilution) | 9 (1x – 4x) | 14 (1x) | 14 (1x) | 1 (1x) | 1 (1x) |
| Repeats/plate, n | 1–4 | 1 | 1 | 2 | 2 |
| Plates per experiment, n | 1 vs 7 | 3 vs 8 | 3 vs 8 | 3 vs 19 | 3 vs 19 |
a
Experiments = analyses done at two different occasions at Olink. Nd = not determined.