Table 5.

Assessment of dilution linearity recovery of the nine synovial fluid control samples (A-I). The analyses were done with or without predefined biomarker selection criteria at different dilution ranges. The data presented in this table was from one experiment (plate) where the samples were diluted and assessed as single sample up to quadruplicates.

		Samples A-D		Samples E-G		Samples H,I
aSelection criteria	Dilutions, range (span)	1–25x (25x)	2–25x (12.5x)	1–10x (10x)	2–10x (5x)	1–4x (4x)
	Sample dilutions	1x, 2x, 4x, 10x, 25x	2x, 4x, 10x, 25x	1x, 2x, 4x, 10x	2x, 4x, 10x	1x, 2x, 4x
	Samples, n	4	4	3	3	2
	Biomarkers
	Nb	19–36	32–41	42–48	42–51	44
	CV % of NPX, mean (range)	11.8 (1.7–20.0)	10.5 (1.3–20.1)	10.9 (2.5–20.2)	10.9 (2.1–19.7)	14.0 (6.4–20.8)
	Range of recovery, %	74.4–126.4	75.5–125.8	75.1–126.0	77.5–120.9	76.7–123.2
		Samples A-D		Samples E-G		Samples H, I
No selection criteria	Dilutions, range (span)	1–25x (25x)	2–25x (12.5x)	1–10x (10x)	2–10x (5x)	1–4x (4x)
	Sample dilution, times	1x, 2x, 4x, 10x, 25x	2x, 4x, 10x, 25x	1x, 2x, 4x, 10x	2x, 4x, 10x	1x, 2x, 4x
	Samples, n	4	4	3	3	2
	Biomarkers
	All, n	92	92	92	92	92
	Values ​< ​LLOD, nc	17–27	17–27	22	22	19, 26
	CV % of NPX, mean (range)	5.2 (1.7–145.5)	43.7 (1.7–145.5)	37.8 (2.5–117)	33.4 (2.5–117)	31.7 (6.4–82.2)
	Range of recovery, %	8.6–358.2	14.2–298.7	10.9–272.6	26.5–216.5	26.4–188.7

NPX ​= ​normalized protein expression.

^aSelection criteria ​= ​values above lower limit of detection (LLOD), coefficient of variation (CV) of linearity <21% and recovery between 74 and 127%.

^bN ​= ​the range of number of biomarkers detected in sample groups at different dilutions. In Supplementary Table S7 the number of biomarkers detected in each sample (A-I) are presented.

^cNumber of biomarker with values < LLOD in individual samples (A-I): 17 (A), 20 (B), 27 (C), 18 (D), 22 (E, F, G), 26 (H) and 19 (I).