Table 6.
Effect of temperature and freeze-thaw treatments on biomarkers in synovial fluid samples. Ratio of NPX-values between temperature or freeze-thaw treated samples vs control (no treatment) are shown. The results are from one experiment and only data from biomarkers that had values above lower limit of quantification are presented.
| 2 h at room temp. |
24 h at 4 °C |
|||
|---|---|---|---|---|
| Undiluted | 4x dilution | Undiluted | 4x dilution | |
| Samples for analyses, n | 3 | 2 | 3 | 2 |
| Biomarkers, n | 70, 76, 78 | 64, 71 | 71, 77, 77 | 64, 73 |
| NPX ratio, mean (range) | 1.03 (0.81–1.72) | 0.99 (0.77–1.28) | 0.95 (0.61–1.36) | 1.05 (0.81–1.65) |
| Biomarkers with NPX ratio between 0.8 and 1.2, n (%) |
67, 72, 76 (96–97) |
63, 69 (98, 97) |
66, 69, 73 (93–95) |
61, 68 (95, 93) |
| 3 cycles |
10 cycles |
|||
| Undiluted |
4x dilution |
Undiluted |
4x dilution |
|
| Samples for analyses, n | 3 | 2 | 2 | 3 |
| Biomarkers, n | 69, 77, 78 | 65, 71 | 70, 77 | 63, 70, 72 |
| NPX ratio, mean (range) | 1.00 (0.73–1.35) | 1.07 (0.85–1.37) | 1.02 (0.83–1.20) | 0.97 (0.70–1.30) |
| Biomarkers with NPX ratio between 0.8 and 1.2, n (%) | 68, 74, 76 (97–99) | 60, 71 (92, 100) | 70, 77 (100, 100) | 61, 69, 70 (97–99) |
NPX = normalized protein expression.