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Effect of PDTC and TPCK on the levels of O. tsutsugamushi-induced chemokine mRNAs in J774A.1 cells. (A) Levels of each chemokine mRNA were analyzed in total RNA samples prepared from uninfected cells (C), O. tsutsugamushi-infected cells (OT), and infected cells in the presence of PDTC (PDTC + OT) or TPCK (TPCK + OT) by RT-PCR analysis. (B) The intensities of bands were determined and normalized as specified for Fig. 2.
