Fig. 4.

Impact of KDM5D and MYBL2 on apoptosis and cell cycling in hormone‐sensitive prostate cancer cells. In this figure from A to D, experiments were performed 3 times. AR, androgen receptor; CSS, charcoal‐stripped serum; DHT, dihydrotestosterone; LV, lentivirus; o.e., overexpression; PrCa, prostate cancer; scr, scramble. (A) Apoptosis activation was monitored in LNCaP and LAPC4 cells using muse apoptosis assay kit. (B) PARP protein levels were determined by western blot of LNCaP and LAPC4 control and knockdown MYBL2. (C) Cell cycle activation was monitored in LNCaP and LAPC4 cells using muse cell cycle. (D) Cell cycle‐related protein levels were determined by western blot of LNCaP and LAPC4. (E) Hypothetical schema showing the target genes of KDM5D/MYBL2 and its effects on prostate cancer networks