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. 2022 Oct 10;16(22):3975–3993. doi: 10.1002/1878-0261.13298

Fig. 5.

Fig. 5

Modulation of THRA‐luc activity by the Wnt agonist and antagonist. Analysis of THRA‐luc and TopFlash activity in the presence of the Wnt agonist CHIR99021 (A) and the Wnt antagonist IWP4 (B) in Caco2, SW480, and HCT116 cells, as indicated. Graphs show the mean ± SD (n = 6) of normalized relative luciferase units (RLU) from at least two independent experiments, each conducted in six replicates. ns, nonsignificant, *P < 0.05, **P < 0.01, and ***P < 0.001 by unpaired, two‐tailed Student's t‐test.