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. 2020 Nov 18;2(4):100124. doi: 10.1016/j.ocarto.2020.100124

Fig. 3.

Fig. 3

Cytokines and gene expression modulated in phC's because of rhuGSN stimulation. Primary human chondrocytes (phCs) were stimulated with different concentrations of rhuGSN for three days (72 h) during cultivation. The supernatant was decanted and used for the ELISA and cells were harvested and used for qPCR. (A) IL-1β production of phCs stimulated. RhuGSN has no significant influence on the production of IL-1β. (B) IL-6 cytokine production in stimulated phCs. Stimulation with 3 μg/mL and increasing concentrations significantly increases the production of IL-6. (C) TNF-α production increases due to rhuGSN stimulation significantly in with 30 μg rhuGSN stimulated phCs and decreases at high concentrations of rhuGSN. (D) TGF-β production shows significant increase due to 3 μg rhuGSN stimulation in phCs and decreases in higher concentrations. (E) Quantitative real-time PCR analysis of cartilage ECM-related genes after treatment with different concentrations of rhuGSN. The treatment of phCs with rhuGSN significantly modulates the expression of cartilage-related genes in vitro. Anova test, n = 4, ∗ <0.5, ∗∗ <0.1 ∗∗∗ <0.05, ∗∗∗∗ <0.001.