Table 3.
Identification of 12 significantly modulated phosphatidylcholines between early- and late-stage OA SF by MRM/MS lipidomic analysis.
| N | Lipid species | m/z | MS mode | eOA (n = 7) | lOA (n = 8) | FC | p-value |
|---|---|---|---|---|---|---|---|
| 1 | D-α-PC dipalmitoyl | 734.60 | POS | 4420.00 ± 1451.00 | 2033.00 ± 743.80 | 2.44 | 0.001 |
| 2 | PC 28:1 | 676.50 | POS | 829.40 ± 389.50 | 322.10 ± 126.70 | 2.58 | 0.003 |
| 3 | PC 30:0 | 706.50 | POS | 1034.00 ± 385.50 | 454.40 ± 147.40 | 2.55 | 0.001 |
| 4 | PC 30:1 | 704.50 | POS | 6789.00 ± 2060.00 | 4169.00 ± 1777.00 | 1.63 | 0.001 |
| 5 | PC 30:2 | 702.50 | POS | 1413.00 ± 539.10 | 684.10 ± 317.10 | 2.07 | 0.004 |
| 6 | PC 32:1 | 732.60 | POS | 4342.00 ± 1465.00 | 2057.00 ± 741.40 | 2.37 | 0.002 |
| 7 | PC 32:2 | 730.50 | POS | 2490.00 ± 683.00 | 1098.00 ± 333.00 | 2.54 | 0.000 |
| 8 | PC 34:1 | 760.60 | POS | 667.30 ± 285.40 | 311.80 ± 80.76 | 2.39 | 0.003 |
| 9 | PC 34:2 | 758.60 | POS | 16386.00 ± 3784.00 | 9784.00 ± 3116.00 | 1.67 | 0.001 |
| 10 | PC 36:0 | 790.60 | POS | 18282.00 ± 5341.00 | 12515.00 ± 3860.00 | 1.46 | 0.031 |
| 11 | PC 36:2 | 786.60 | POS | 2682.00 ± 1084.00 | 1354.00 ± 621.90 | 1.98 | 0.011 |
| 12 | PCaa 24:0 | 622.44 | POS | 16992.00 ± 4246.00 | 11444.00 ± 3658.00 | 1.48 | 0.017 |
eOA: early-stage osteoarthritis; lOA: late-stage osteoarthritis; POS: positive ion mode; FC: fold change; PC: phosphatidylcholine.
∗significant difference from control SF.
#significant difference between eOA and lOA.