Fig. 2.
Acute exercise results in distinct metabolomic profiles. (A) Acute exercise alters the cumulative metabolite distributions. Mirrored metabolite feature distributions of m/z values and corresponding intensities were plotted to illustrate any differences in distributions. KS test showed a statistically significant (pks<0.01) change in metabolite distributions comparing control to acute exercised SF. (B) Unsupervised hierarchical cluster analysis illustrates unsupervised clustering of most exercised (red line) and control samples (blue line). Tree distances are represented as Euclidean distances. Distances run by exercised mice shown on the right. Tree distances are represented as Euclidean distances. Red represents exercised SF and blue represents control SF. (C) Clustergram representation of all metabolite features detected shows overall metabolomic profiles of each sample. Clustering of samples is illustrated by the dendrogram on the top, and clustering of co-metabolites is illustrated by the dendrogram on the side. (D) Supervised PLSA-DA clearly discriminated between acute exercised and control samples. PLS-DA score plot illustrated separation between of exercised and control samples, with PC1 containing 13.2% of the variation and PC2 containing 14.5% of the variation.