Table 2.
Mechanosensitive metabolites identified by pairwise biserial correlation coefficient analysis. Correlation coefficient analysis identified metabolite features that were accumulated or depleted after acute exercise. Strongly correlated features were matched with metabolite identities using METLIN (adducts: H+, Na+; mass tolerance: 15 ppm). False positives and drugs were removed from compound matches. An uncondensed list of compound matches is shown in Supplemental Table 5. Mass-to-charge ratios may match multiple metabolite identities because of either isoptomers or differential adduct formation (H+ or Na+) during ionization. Columns: Metabolite identity-putative metabolite match between experimental data and Metlin database. Mass-mass of putative metabolite. PPM—difference between database and experimental metabolite in parts per million. Adduct—ion adduct of experimental metabolite feature. Feature—mass of experimental metabolite feature. Coefficient—correlation coefficient. Accumulated/Depleted—indicates if the metabolite increased or decreased with exercise.
| MECHANOSENSITIVE METABOLITE FEATURES | ||||||
|---|---|---|---|---|---|---|
| Correlation Coefficient Analysis | ||||||
| Metabolite Identity | Mass | PPM | Adduct | Feature | rpb | P-value |
| 491.4027405 | −0.992 | 0.008 | ||||
| 618.4603464 | −0.981 | 0.019 | ||||
| Phosphatidylcholine | 591.39 | 9 | Na | 614.3850952 | −0.969 | 0.006 |
| 755.0263367 | −0.967 | 0.033 | ||||
| Phosphatidylserine | 511.3274 | 11 | Na | 534.3226522 | −0.963 | 0.002 |
| 928.7206607 | −0.960 | 0.040 | ||||
| 6-Monoacetyl morphine-3-O-glucuronide | 373.2406 | 0 | H | 374.2474714 | −0.959 | 0.002 |
| Androsta-5,16-dienol [17,16-b] quinolin-3beta-ol | 373.2406 | 0 | ||||
| Dodecylphosphocholine | 351.2538 | 11 | Na | |||
| 2,3-dinor-6-keto Prostaglandin F1α-d9 | 351.2607 | 6 | ||||
| 141.9570389 | −0.956 | 0.011 | ||||
| 491.8885193 | −0.954 | 0.046 | ||||
| 686.3826643 | −0.952 | 0.003 | ||||
| 250.1348 | 0.929 | 0.023 | ||||
| 342.2328 | 0.934 | 0.020 | ||||
| Vitamin D3 derivative | 522.3764 | 1 | H | 545.3847 | 0.944 | 0.056 |
| 697.9982 | 0.948 | 0.052 | ||||
| 328.2565 | 0.949 | 0.004 | ||||
| Phenylglyoxylyl-CoA | 899.1363 | 11 | H | 900.1329 | 0.961 | 0.039 |
| 3,4-didehydroadipyl-CoA semialdehyde | 877.152 | 9 | Na | |||
| 328.2696 | 0.966 | 0.007 | ||||
| Phosphatidyserine | 899.6615 | 9 | H | 900.6772 | 0.973 | 0.027 |
| Phosphatidylcholine | 877.6924 | 4 | Na | |||
| 359.2435 | 0.973 | 0.005 | ||||
| Hydroxypentobarbital | 242.1267 | 2 | H | 243.133316 | 0.976 | 0.024 |
| 2,6-di-tert-butylbenzoquinone | 220.1463 | 9 | Na | |||
| 3-Phenylpropyl isovalerate | 220.1463 | 9 | ||||
| 13-Nor-6-eremophilene-8,11-dione | 220.1463 | 9 | ||||
| Hexyl phenylacetate | 220.1463 | 9 | ||||
| 2-Phenylethyl hexanoate | 220.1463 | 9 | ||||
| Tetradecene-1,3-diyne-diol derivatives | 220.1463 | 9 | ||||
| 2-Methyl-1-phenyl-2-propanyl butyrate | 220.1463 | 9 | ||||