Table 3.
Mechanosensitive metabolites identified by volcano plot analysis. Mechanosensitive features upregulated or downregulated with exercise were identified by volcano plot analysis. Features with a greater than twofold change and p-value (prior to FDR correction) less than 0.05 were considered mechanosensitive metabolites. Metabolite identities were assigned via METLIN (adducts H+, Na+; mass tolerance: 15 ppm). False positives and drugs were removed from compound matches. An uncondensed list of compound matches is shown in Supplemental Table 6. Mass-to-charge ratios may match multiple metabolite identities because of either isoptomers or differential adduct formation (H+ or Na+) during ionization. Columns: Metabolite identity-putative metabolite match between experimental data and Metlin database. Mass-mass of putative metabolite. PPM—difference between database and experimental metabolite in parts per million. Adduct—ion adduct of experimental metabolite feature. Feature—mass of experimental metabolite feature. Fold Change—fold change of metabolite between exercise and control group. P-value—FDR-corrected p-value for comparing between exercise and control. Accumulated/Depleted—indicate if expression increased or decreased with exercise.
| MECHANOSENSITIVE METABOLITE FEATURES | |||||||
|---|---|---|---|---|---|---|---|
| Volcano Plot Analysis | |||||||
| Metabolite Identity | Mass | PPM | Adduct | Feature | Fold Change | P-value | Accumulated (↑)/Depleted (↓) |
| 540.3787 | 0.07398 | 0.002015 | ↓ | ||||
| 724.3843 | 3.0682 | 0.002631 | ↑ | ||||
| 432.3288 | 14.191 | 0.006931 | ↑ | ||||
| 254.1660 | 4.7594 | 0.007132 | ↑ | ||||
| alpha-licanic acid | 292.2038 | 11 | Na | 315.1893 | 2.2216 | 0.007407 | ↑ |
| Colnelenic acid | 292.2038 | 11 | |||||
| Etherolenic acid | 292.2038 | 11 | |||||
| Octadecatrienoic acid derivatives | 292.2038 | 11 | |||||
| Oxo-phytodienoic acid derviatives | 292.2038 | 11 | |||||
| 588.3495 | 6.6728 | 0.01123 | ↑ | ||||
| Phosphatidylserine | 573.3067 | 13 | H | 574.3215 | 2.8375 | 0.01366 | ↑ |
| 3-hydroxyphenytoin | 268.0848 | 5 | H | 269.0934 | 3.4668 | 0.01427 | ↑ |
| 2,4-Imidazolidinedione, 5-(7-oxabicyclo [4.1.0]-hepta-2,4-dien-3-yl)-5-phenyl- | 268.0848 | 5 | |||||
| 2,3-Dihydroxycarbamazepine | 268.0848 | 5 | |||||
| Benzo [a]pyrene derivatives | 268.0888 | 9 | |||||
| Cysteinyl-phenylalanine | 268.0882 | 7 | |||||
| Phenylalanyl-cysteine | 268.0882 | 7 | |||||
| N-acetyl-tryptophan | 246.1004 | 14 | Na | ||||
| methionyl-proline | 246.1038 | 1 | |||||
| prolyl-methionine | 246.1038 | 1 | |||||
| 389.2768 | 4.4307 | 0.02512 | ↑ | ||||
| 416.2059 | 2.1272 | 0.02681 | ↑ | ||||
| 158.9622 | 5.6077 | 0.02872 | ↑ | ||||
| 574.2965 | 2.1147 | 0.02902 | ↑ | ||||
| idebenone metabolite (QS-4) | 268.0947 | 10 | H | 269.1048 | 3.6993 | 0.03387 | ↑ |
| diethyl 2,6-dimethyl-4-oxo-4h-pyran-3,5-dicarboxylate | 268.0947 | 10 | |||||
| 3,3′-diindolylmethane | 246.1157 | 0 | Na | ||||
| Unoprostone isopropyl ester | 424.3189 | 8 | H | 425.3225 | 0.2154 | 0.03620 | ↓ |
| 4,4′-Diaponeurosporene | 402.3287 | 10 | Na | ||||
| fluorovitamin D3/fluorocholecalciferol derivatives | 402.3298 | 8 | |||||
| 25-Fluorovitamin D3 | 402.3298 | 8 | |||||
| Thiocholesterol | 402.3298 | 8 | |||||
| Phosphatidylcholine | 677.4996 | 12 | H | 678.4983 | 0.3988 | 0.03687 | ↓ |
| Phosphatidylethanolamine | 677.4995 | 12 | |||||
| 544.3291 | 3.9614 | 0.04001 | ↑ | ||||
| 608.3555 | 0.1292 | 0.04109 | ↓ | ||||
| 598.4739 | 0.2565 | 0.04653 | ↓ | ||||
| 291.2023 | 5.8616 | 0.04775 | ↑ | ||||
| 716.9248 | 2.4243 | 0.04794 | ↑ | ||||
| 782.4581 | 8.2303 | 0.04807 | ↑ | ||||
| Phosphatidylcholine | 523.4002 | 6 | Na | 546.3927 | 0.2842 | 0.04888 | ↓ |
| 2-o-methyl PAF C18 | 523.4002 | 6 | |||||