Table 2.
Fluorochrome conjugated antibodies used for multichromatic flow cytometry.
| Marker | Antibody Clone | Isotype control | Fluorochrome |
|---|---|---|---|
| ISCTaMSC markers | |||
| CD73 | AD2 | Mouse IgG1 | BV421 |
| CD90 | 5E10 | Mouse IgG1 | PE |
| CD105 | 266 | Mouse IgG1 | APC |
| CD14 | MϕP9 | Mouse IgG2b | PercP-Cy5.5 |
| CD19 | HIB19 | Mouse IgG1 | BV421 |
| CD34 | 581 | Mouse IgG1 | APC |
| CD45 | HI30 | Mouse IgG1 | PE |
| HLA-DR | TU36 | Mouse IgG2b | APC |
| Chondropotency markers | |||
| CD44 | G44-26 | Mouse IgG2b | PercP-Cy5.5 |
| CD166 | 3A6 | Mouse IgG1 | BV421 |
| CD49c | C3 II.1 | Mouse IgG1 | PE |
| CD106 | 51-10C9 | Mouse IgG1 | APC |
| CD151 | 14A2.H1 | Mouse IgG1 | PE |
| CD39 | TU66 | Mouse IgG2b | APC |
| CD271 | C40-1457 | Mouse IgG1 | BV421 |
| Macrophage markers | |||
| CD14 (pan marker) | M5E2 | Mouse IgG2a | APC |
| CD80 (M1) | L307.4 | Mouse IgG1 | PE |
| CD86 (M1) | 2331 (FUN-1) | Mouse IgG1 | FITC |
| CD206 (M2) | 19.2 | Mouse IgG1 | BV421 |
| CD163 (M2) | GHI/61 | Mouse IgG1 | PerCP-Cy5.5 |
a
ISCT: International Society for Cellular Therapy.