DMEM GlutaMAX (high glucose) |
98% |
89% |
Gibco 61965 |
Antibiotics |
10 U/mL penicillin, 10 μg/mL streptomycin, 0.25 μg/mL amphotericin (1% (v/v)) |
10 U/mL penicillin, 10 μg/mL streptomycin, 0.25 μg/mL amphotericin (1% (v/v)) |
Gibco, 15240 |
Sodium-pyruvate |
1 mM |
– |
Gibco, 11360 |
l-ascorbic acid-2-phosphate |
50 μg/mL |
50 μg/mL |
Wako chemicals, 013-19641 |
L-proline |
40 μg/mL |
– |
Sigma-Aldrich, P5607 |
Dexamethasone |
100 nM |
100 nM |
Sigma-Aldrich, D4902 |
Fetal bovine serum (FBS) |
|
10% (v/v) |
Gibco, 10270106 |
Insulin-transferrin-selenin |
1% (v/v) |
– |
ITS+-premix, Gibco, Life Technologies, 32430 |
β-glycerophosphate |
– |
10 mM |
Sigma-Aldrich, G9422 |