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. 2021 Apr 29;3(2):100173. doi: 10.1016/j.ocarto.2021.100173

Table 1.

Composition of bone and cartilage media for ex vivo culture of osteochondral explants.

Cartilage media Bone media Supplier
DMEM GlutaMAX (high glucose) 98% 89% Gibco 61965
Antibiotics 10 ​U/mL penicillin, 10 ​μg/mL streptomycin, 0.25 ​μg/mL amphotericin (1% (v/v)) 10 ​U/mL penicillin, 10 ​μg/mL streptomycin, 0.25 ​μg/mL amphotericin (1% (v/v)) Gibco, 15240
Sodium-pyruvate 1 ​mM Gibco, 11360
l-ascorbic acid-2-phosphate 50 ​μg/mL 50 ​μg/mL Wako chemicals, 013-19641
L-proline 40 ​μg/mL Sigma-Aldrich, P5607
Dexamethasone 100 ​nM 100 ​nM Sigma-Aldrich, D4902
Fetal bovine serum (FBS) 10% (v/v) Gibco, 10270106
Insulin-transferrin-selenin 1% (v/v) ITS+-premix, Gibco, Life Technologies, 32430
β-glycerophosphate 10 ​mM Sigma-Aldrich, G9422