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. Author manuscript; available in PMC: 2022 Dec 2.
Published in final edited form as: Cancer Gene Ther. 2020 Jul 7;28(1-2):98–111. doi: 10.1038/s41417-020-0189-4

Figure 4. OV-induced TILs can be cultured and expanded ex vivo and retain their tumor specificity.

Figure 4.

(A). Tumor specificity of the expanded TILs. TILs from each individual mouse have been cultured for 4 days and then tested by IFN-γ ELISPOT assay. (B, C). Analysis of 4–1BB upregulation on (B) CD4+ and (C) CD8+ T cells by flow cytometry. As previously described, T cells were either left unstimulated (medium) or challenged with γ-irradiated MC38 tumor cells or γ-irradiated B16 tumor cells or naïve splenocytes from non-tumor-bearing B6 mouse in duplicate. After 24 h the cells have been stained for flow cytometry analysis for CD3, CD4, CD8, 4–1BB. Results are shown as individual data points (percentage of CD8+4–1BB+ T cells and CD4+4–1BB+ T cells) and bars (means ± standard deviation) of T cells from each mouse evaluated in duplicate. Data are presented as summary from 2 out of 5 independent experiments (n = 3–4 mice/group). For multiple group comparison One-way ANOVA was used. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.