Fig 1. General characteristics of fixed genomic variants that emerged during CZA selection.

(A) Proportions and types of fixed variants acquired during the course of CZA adaptation for each genotype. The total number of fixed variants acquired across lineages per genotype is indicated in parenthesis. (B) Evolutionary conservation of genes with fixed variants acquired in MPAO1-mutS^Tn- (yellow) and MPAO1-WT (red) CZA adaptation experiments in the Pseudomonas genus. Each P. aeruginosa PAO1 CDS is plotted as a dot representing average amino acid identity and the proportion of genomes containing the given CDS (breadth of coverage) among a set of 100 complete Pseudomonas genomes (see S6 Data and Methods). Genes without fixed mutation are represented in blue. The marginal density plots and histograms show the distribution of a corresponding conservation measure in all CDSs (gray) and genes containing fixed variants (blue), respectively. The underlying data to generate this figure can be found in S2 Data. CZA, ceftazidime/avibactam; WT, wild type.