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Larvae were orally infected with W22703, W22703 ΔtcaA, W22703 ΔtcaA/pACAC-tcaA, W22703 ΔHE, W22703 ΔHE/pACYC-HE, W22703 ΔtccC, and W22703 ΔtccC/pBAD-tccC. Six animals per time-point were used, and the experiments were performed as triplicates, resulting in a total of 18 larvae per time-point per strain. Larvae fed with LB were used as a negative control. Standard deviations are indicated as error bars.
