FIG. 1.

Cloning of papLe22 cDNA. (a) PCR product of the λgt11 clone 9C amplified with λgt11 primers (lane 1) and RACE-PCR products of L. infantum cDNA amplified with miniexon and R1 primers (lane 2) or miniexon and R2 primers (lane 3) were electrophoresed on 1.5% (lane 1) or 1% (lanes 2 and 3) agarose gels containing 0.5 μg of ethidium bromide/ml. DNA length markers are indicated in base pairs. (b) Amino acid sequence of papLe22 (top) and diagrammatic representation of the 876-bp papLe22 5′ cDNA (bottom). The 594-bp ORF is between the ATG initiation and TAG stop codons. Hatched box, sequence of the λgt11 clone 9C (5′ end at nucleotide 258 of the ORF); arrows, PCR primers; segments, 5′ restriction enzyme sites.