Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Mettl3 | GenBank | ID: 56335 | |
| Genetic reagent (M. musculus) | Mettl3floxed | Lin et al., 2017 | ||
| Genetic reagent (M. musculus) | Six3-Cre | Furuta et al., 2000 | ||
| Genetic reagent (M. musculus) | Rlbp1-GFP | Vázquez-Chona et al., 2009 | ||
| Antibody | Anti-METTL3 (rabbit monoclonal) | Abcam | Cat# ab195352; RRID:AB_2721254 |
1:500 |
| Antibody | Anti-RECOVERIN (rabbit polyclonal) | Millipore | Cat# AB5585; RRID:AB_2253622 |
1:1000 |
| Antibody | Anti-VSX2 (sheep polyclonal) | Millipore | Cat# AB9014; RRID:AB_262173 |
1:500 |
| Antibody | Anti-SOX9 (rabbit polyclonal) | Millipore | Cat# ABS571; RRID:AB_2783876 | 1:500 |
| Antibody | Anti-CALBINDIN (rabbit polyclonal) | Swant | Cat# CB38; RRID:AB_10000340 |
1:500 |
| Antibody | Anti-SYNTAXIN (mouse monoclonal) | Sigma | Cat# S0664; RRID:AB_477483 |
1:200 |
| Antibody | Anti-BRN3A (mouse monoclonal) | Millipore | Cat# MAB1585; RRID:AB_94166 |
1:100 |
| Antibody | Anti-GFP (chicken polyclonal) | Abcam | Cat# ab13970; RRID:AB_300798 |
1:2000 |
| Antibody | Anti-N-CADHERIN (rabbit polyclonal) | Santa Cruz | Cat# sc-7939; RRID:AB_647794 |
1:1000 |
| Antibody | Anti-GS (mouse monoclonal) | BD Biosciences | Cat# 610518; RRID:AB_397880 |
1:2000 |
| Antibody | Anti-GFAP (mouse monoclonal) | Sigma | Cat# G3893; RRID:AB_477010 |
1:2000 |
| Antibody | Anti-BrdU (mouse monoclonal) | GE Healthcare | Cat# RPN202 | 1:50 |
| Antibody | Anti-pH3 (mouse monoclonal) | Cell Signaling | Cat# 9706; RRID:AB_331748 |
1:2000 |
| Antibody | Anti-KI67 (rabbit polyclonal) | Abcam | Cat# ab15580; RRID:AB_443209 |
1:500 |
| Antibody | Alexa Fluor 488 donkey anti-mouse (donkey polyclonal) | Thermo Fisher | Cat# A21202 | 1:1000 |
| Antibody | Alexa Fluor 568 donkey anti-mouse (donkey polyclonal) | Thermo Fisher | Cat# A10037 | 1:1000 |
| Antibody | Alexa Fluor 488 donkey anti-rabbit (donkey polyclonal) | Thermo Fisher | Cat# A21206 | 1:1000 |
| Antibody | Alexa Fluor 568 donkey anti-rabbit (donkey polyclonal) | Thermo Fisher | Cat# A10042 | 1:1000 |
| Antibody | Alexa Fluor 488 donkey anti-chicken (donkey polyclonal) | Jackson ImmunoResearch | Cat# 703-296-155 | 1:1000 |
| Recombinant DNA reagent | pCAGIG (plasmid) | Addgene | Cat# 11159 | CDS overexpression |
| Recombinant DNA reagent | pSicoR (plasmid) | Addgene | Cat# 11579 | shRNA expression |
| Sequence-based reagent | Rsp14 forward | This paper | MeRIP-qPCR primers | acctggagcccagtcagccc |
| Sequence-based reagent | Rsp14 reverse | This paper | MeRIP-qPCR primers | cacagacggcgaccacgacg |
| Sequence-based reagent | Pax6 forward | This paper | MeRIP-qPCR primers | actctgccaatgactatgtg |
| Sequence-based reagent | Pax6 reverse | This paper | MeRIP-qPCR primers | ctccagttcaggacagttac |
| Sequence-based reagent | Sox2 forward | This paper | MeRIP-qPCR primers | ctggactgcgaactggagaa |
| Sequence-based reagent | Sox2 reverse | This paper | MeRIP-qPCR primers | actctcctctttttgcaccc |
| Sequence-based reagent | Rax forward | This paper | MeRIP-qPCR primers | ggaaattcagcctcgctgtc |
| Sequence-based reagent | Rax reverse | This paper | MeRIP-qPCR primers | ccaggtcaagatccttggtc |
| Sequence-based reagent | Zfp292 forward | This paper | MeRIP-qPCR primers | gggaaataacgaatttcagg |
| Sequence-based reagent | Zfp292 reverse | This paper | MeRIP-qPCR primers | ctctcttcaaattaccaggc |
| Sequence-based reagent | Ckap4 forward | This paper | MeRIP-qPCR primers | ggaacgacctggataggttg |
| Sequence-based reagent | Ckap4 reverse | This paper | MeRIP-qPCR primers | cgtaagaaactgtgcccacac |
| Sequence-based reagent | Traf4 forward | This paper | MeRIP-qPCR primers | ggatgatgcggttttcatccg |
| Sequence-based reagent | Traf4 reverse | This paper | MeRIP-qPCR primers | ccagtttcagatccagtcccg |
| Sequence-based reagent | Bcl7a forward | This paper | MeRIP-qPCR primers | ccaagaagaacctagagcgg |
| Sequence-based reagent | Bcl7a reverse | This paper | MeRIP-qPCR primers | ggcagtcacttgaaggttcg |
| Sequence-based reagent | Rap2b forward | This paper | MeRIP-qPCR primers | ggacttggagggtgaacgtga |
| Sequence-based reagent | Rap2b reverse | This paper | MeRIP-qPCR primers | gatgtctccatgaaggggcag |
| Sequence-based reagent | Nxt1 forward | This paper | MeRIP-qPCR primers | ccagtaacacggtgtggaag |
| Sequence-based reagent | Nxt1 reverse | This paper | MeRIP-qPCR primers | gagactggcatttctctgcag |
| Sequence-based reagent | Apex1 forward | This paper | MeRIP-qPCR primers | gttgggatgaagccttcc |
| Sequence-based reagent | Apex1 reverse | This paper | MeRIP-qPCR primers | catgagccacattgagatcc |
| Sequence-based reagent | Sulf2 forward | This paper | MeRIP-qPCR primers | ctttggagaaagcacggac |
| Sequence-based reagent | Sulf2 reverse | This paper | MeRIP-qPCR primers | cttctgagccagccaggtc |
| Other | Mettl3 | GenBank | CGTCAGTATCTTGGGCAAATT | shRNA targeting site |
| Other | Zfp292 | GenBank | TGTGGCAGTAAGCCATATATA | shRNA targeting site |
| Other | Ckap4 | GenBank | ACGACCTGGATAGGTTGTTTC | shRNA targeting site |
| Other | Traf4 | GenBank | GCGTATAGTTCCCACTAATTT | shRNA targeting site |
| Other | Bcl7a | GenBank | CGGAGCCAAAGGTTGATGATA | shRNA targeting site |