Figure 2.

T cell responses against Orthopox MPs after vaccinia vaccination; refers to Figures S1 and S2 and Table S1. List of orthopox IEDB epitopes, related to Figures 1 and 3, Table S4. List of predicted MPX CD4 and CD8 epitopes, related to Figures 1 and 4

PBMCs from each time point were tested in AIM/ICS assays with experimentally defined OPXV MPs, OP-CD4-E (blue), and OP-CD8-E (red).

(A and B) OP-CD4-E-specific CD4 ⁺ T cell reactivity is shown for (A) AIM and (B) ICS.

(C and D) OP-CD8-E specific CD8 ⁺ T cell reactivity is shown for (C) AIM and (D) ICS. The y axis of each bar graph starts at the limit of detection (LOD), and the limit of sensitivity (LOS) is indicated with a dotted line. Columns represent the geometric mean and error bars indicate the geometric standard deviation. Pie charts below each bar indicate the frequency of positive responders. Mann-Whitney t test was applied to each graph, and p values symbols are shown when significant. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001. X² test was applied to the frequencies of positivity, and p values are listed on the right of each graph.