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. 2022 Jun 22;27(10):4252–4263. doi: 10.1038/s41380-022-01656-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A–C Immunoperoxidase labeling for GCPII in aged macaque (28–30 y) dlPFC layer III is visualized within astroglial leaflets ensheathing asymmetric (likely glutamatergic) synapses. GCPII immunolabeling appears to show a preference for the astroglial leaflets (PAPs) near the plasma membrane in perisynaptic locations near the excitatory glutamatergic synapse. D–G GCPII immunolabeling is prominently expressed in postsynaptic compartments in dendritic spines, near the plasma membrane and PSD synaptic active zone (D, F and G). Intriguingly, GCPII immunolabeling is visualized in vesicular-like structures within the dendritic spine head, positioned to undergo endocytosis or exocytosis (E and F). In (G), a dendritic spine is seen emanating from the dendritic shaft of a pyramidal cell; both are immunopositive for GCPII. Sparse presynaptic GCPII labeling is also observed in the axon terminal in association with mitochondria. All dendritic spines receive axospinous Type I asymmetric glutamatergic-like synapses, and the spine apparatus is pseudocolored in pink in the spine head. Synapses are between arrows. H–I In aged macaque dlPFC layer III, GCPII immunolabeling was visualized in dendritic shafts and was associated with microtubules oriented in parallel bundles. In (I), the dendritic shaft receives an axodendritic symmetric Type II synapse (arrowheads) and GCPII immunolabeling within the dendritic shaft was in close proximity to mitochondria. Color-coded arrowheads (magenta) point to GCPII immunoreactivity. Profiles are pseudocolored for clarity. Ax axon, Mit mitochondria, Sp dendritic spine, As astroglia, Den dendrite. Scale bars: 200 nm.