Table 1.
Mass spectral analysis of native and thio-linked hemi-A heparosan
| Product | GlcNAc-R’ | (GlcNAc-GlcA)1-GlcNAc-R’ | (GlcNAc-GlcA)2-GlcNAc-R’ |
|---|---|---|---|
| Native (= 4-OH) | |||
| Observed | 950.38 ± 0.11 | 1,329.50 ± 0.04 | 1,708.65 ± 0.05 |
| Predicted | 950.29 | 1,329.40 | 1,708.51 |
| 4-SH Analog | |||
| Observed | 966.29 ± 0.11 | 1,361.43 ± 0.05 | 1,756.56 ± 0.06 |
| Predicted | 966.27 | 1,361.36 | 1,756.44 |
| Observed ∆ S-O | 15.91 | 31.93 | 47.91 |
The observed and predicted masses (Da) are shown. The mass difference between the natural O-linked and the artificial S-linked species (last row of table) corresponds to the additional mass (~16 Da per S atom) associated with the replacement of oxygen with 1, 2, or 3 sulfur atoms (acceptor R’ = GlcA-GlcNAc-GlcA-C2H4-amido-benzaldehyde).