Figure 4.

Alterations in Ob-Rb in the BAT of TDP-43^A315T mice in response to O₃ exposure. (A) Leptin and (B) Ob-Rb mRNA expression was assessed by RT-qPCR in the BAT of TDP-43^A315T mice exposed to FA (n = 5 mice) or O₃ (n = 7 mice) compared to WT controls exposed to FA (n = 6 mice) or O₃ (n = 8 mice) at the end-stage of disease. Values are expressed as the mean ± SEM for the different groups. Comparison between groups was performed by two-way ANOVA followed by Dunnett´s post hoc test to compare all groups with WT-FA, while Tukey’s post hoc test was used for multiple comparisons between all groups, where * p < 0.05 vs. WT mice FA-exposed; # p < 0.05 vs. WT mice O₃-exposed; ** p < 0.05 vs. TDP-43^A315T FA-exposed. Abbreviations: WT, Wwild-type mice; TDP-43, TDP-43^A315T mice; FA, filtered air; O₃ , ozone; BAT, brown adipose tissue.