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. 2022 Dec 2;12:20814. doi: 10.1038/s41598-022-25033-4

Table 2.

Primers used for RT-qPCR of genes involved in the metabolism of BAT.

Gene name Forward primer Reverse primer
Prdm16 5′-ATGGGCTTTGACCATACCCG-3′ 5′-TCGGCTCCAAAGCTAACAGG-3′
PGC1α 5′-GCACGCAGCCCTATTCATTG-3′ 5′-TGAGTCTCGACACGGAGAGT-3′
PPARγ 5′-TGAAAGAAGCGGTGAACCACTG-3′ 5′-TGGCATCTCTGTGTCAACCATG-3′
AdipoQ 5′-TCCCAATGTACCCATTCGCT-3′ 5′-AGAGTCCCGGAATGTTGCAG-3′
Fabp4 5′-CCTTCAAACTGGGCGTGGAA-3′ 5′-CCCCGCCATCTAGGGTTATG-3′
GLUT4 5′-GCTCTGACCGATGGGGAACC-3′ 5′-AAACTGAAGGGAGCCAAGCA-3′
UCP1 5′-AGTACCCAAGCGTACCAAGC-3′ 5′-GACCCGAGTCGCAGAAAGA-3′
Leptin 5′-TTCACACACGCAGTCGGTAT-3′ 5′-GAAGTCCAAGCCAGTGACCC-3′
Ob-Rb 5′-CCCCCACTGAAAGACAGCTT-3′ 5′-TTCACATCCCCGAAGACTGC-3′