Fig. 5. The number of LAT condensates correlates with ligand potency.

a TIRF images over time of primary mouse T cells deposited onto supported membranes containing 600 molecules μm⁻² ICAM-1 and one of four distinct peptides at the indicated concentrations: MCC(Atto647), T102S(Atto647), ER60(Atto647), or T102E. b cumulative time-series for the number of distinct LAT condensates observed over a sample of cells. From most productive to least: MCC (red), T102S (blue), ER60 (green), and lastly T102E (gray). Compare to Supplementary Fig. 2g. The mean was plotted as a solid dark line, while the lighter-colored error bands were derived from $\pm$1 standard deviation. Scale bar is 5 μm. Source data are provided as a Source data file.