Skip to main content
. 2022 Dec 3;13:7463. doi: 10.1038/s41467-022-35183-8

Fig. 4. Matrigel HDB group promotes MSCs proliferation and migration through the PI3K-Akt signaling pathway and Wnt/β-CATENIN signaling pathway.

Fig. 4

a Venn diagram showing the differentially expressed genes (DEGs) in the Matrigel HDG group and the Matrigel LDB group, both compared to the Matrigel HDB group. b and c Volcano diagram of DEGs in the Matrigel HDG group (b) and the Matrigel LDB group (c), both compared to the Matrigel HDB group. d Heatmap view of the overlapped 1328 DEGs in the Matrigel HDG group and the Matrigel LDB group, both compared to the Matrigel HDB group. e KEGG pathway analysis of the 1328 DEGs. Significant differential genes were determined using a one-sided wald test in the DESeq2 package. f Heatmap view of 30 DEGs belonging to the PI3K-Akt pathway. g Western blotting of PI3K–Akt signaling pathway proteins, pAKT and AKT, and their downstream proteins, including pGSK3β, GSK3β, β-CATENIN. Samples were derived from the same experiment and the gels were processed in parallel. h Immunofluorescence for pAKT, AKT, and β-CATENIN. Scale bar, 200 µm. i Schematic elaboration of the PI3K–AKT and Wnt/β-CATENIN signaling pathways promoting MSCs migration and proliferation.