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. 2022 Dec 3;13:7463. doi: 10.1038/s41467-022-35183-8

Fig. 7. Investigation of myofiber maturation, muscle resident cells, angiogenesis, neural integration, and MSCs immunomodulatory effects in regenerated muscle tissues.

Fig. 7

a, b Immunofluorescence for desmin (a) and MYH7 (b) to determine the myofiber maturation and their quantification by fluorescence area. c Immunofluorescence for PAX7 to determine the population of muscle resident cells and their quantification by fluorescence area. d and e Immunofluorescence for CD31 (d) and α-SMA (e) and to evaluate angiogenesis. α-SMA positive arterioles and CD31 positive capillaries are quantified by their fluorescence area. f and g Immunofluorescence for neurofilament (NF) (f) and βIIIT (g) to observe the neural integration. Neurofilament amount and βIIIT magnitude are quantified by their fluorescence area. h-i, Immunofluorescence for CD68 (macrophage) (h) and CD3 (T-cells) (i) to explore the MSCs immunomodulatory effects in muscle regeneration. The signal intensity of CD68 and CD3 were quantified by their fluorescence area. Scale bar, 100 µm (a, b, e), 40 µm (c, d, h, i), 20 µm (f, g). All data are represented as mean ± SD. n = 6 per group at each time point. For each data, at least six imaging windows are randomly selected. Significant difference is determined by one-way ANOVA, followed by Tukey’s test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.