Fig. 4. Pan-endothelial COUP-TFII expression induces ectopic Madcam1 and St6gal1 in intestinal capillaries.
A Experimental timeline for induction of COUP-TFII in EC. B UMAP plot of PP BEC colored by EC subset (left) or by genotype (right). C Violin plots showing expression of select genes in EC-iCOUPOE vs control BEC subsets. Dots represent mean gene expression of separate cohorts (male and female cohorts from two independent experiments), and are presented with SEM. D Confocal imaging showing MAdCAM1 expression in lamina propria capillaries in EC-iCOUPOE (arrowheads) but not control (arrows) mice. Scale bars: 50 µm, EC stained by i.v. injection of directly conjugated antibodies. 20×, whole mount. See also Supplementary Fig. 6A for MAdCAM1 extension into capillary segments in PP.