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. 2022 Dec 2;13:7414. doi: 10.1038/s41467-022-35199-0

Fig. 2. Translocation of TCA cycle enzymes in primed-to-naive transition and totipotency stem cells.

Fig. 2

a, b Immunostaining of Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha, and Mdh2 (purple) in mouse epiblast stem cells (EpiSCs) (a) or OG2 embryonic stem cells (naive stem cells) (b). The mito-DsRed (Mito) was used as a marker of mitochondria. Oct4::GFP is an indicator of embryonic stem cells and DAPI (blue) for indicating the nucleus. Scale bars, 5 µm. c The nuclei/mitochondria fluorescent (N/M) ratio of TCA cycle enzymes in EpiSCs and OG2 ESCs (Naive). Data are presented as the mean ± S.D of at least 30 cells from three independent experiments. A two-tailed unpaired Student’s t test was used. d, Immunostaining of Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha, and Mdh2 (purple) in 2-cell stage-like cells (2CLCs). Oct4::GFP was used to track embryonic stem cells, 2C::tdTomato (red) was used to track 2CLCs, and DAPI (blue) for indicating the nucleus. Scale bars, 5 µm. Three independent experiments were repeated with similar results and one representative picture was presented (a, b, d).