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. 2022 Dec 2;13:7414. doi: 10.1038/s41467-022-35199-0

Fig. 5. Nuclear-localized Pdha1 promotes histone acetylation of pluripotent genes by facilitating the enrichment by P300.

Fig. 5

a Pileup (upper panel) and heatmap (lower panel) of H3K9ac, H3K27ac ChIP-seq near the TSS in MEFs transduced with SKOM plus nls-Pdha1 or Flag on day 4. 2 independent experiments were repeated with similar results, and one replicate (n = 1, each group) was used for ChIP-seq (upstream 3 kb and downstream 3 kb of the TSS). b GO analysis for the genes with >1.5-fold enrichment of H3K9ac and H3K27ac in the nls-Pdha1 overexpression group at day 4 during somatic cell reprogramming. c Global view of H3K9ac and H3K27ac levels at genes with >1.5-fold upregulated normalized tag density near the TSS at day 4. A total of 3571 genes were contained. Two independent experiments were repeated with similar results, and one replicate (n = 1, each group) was used for this analysis. d Venn diagram depicting overlap of the genes where both H3K9ac and H3K27ac increased around TSS sites with genes upregulated in somatic cell reprogramming. A total of 3571 genes were contained in ChIP-seq data, while a total of 2779 genes were contained in RNA-seq data. e Dynamic of the overlayed genes (d) in SKOM induced reprogramming with or without nls-Pdha1 or Flag. 2 independent experiments were repeated with similar results, and one replicate (n = 1, each group) was used for this analysis (b–e). f ChIP-qPCR analysis of P300 enrichment on promoter regions of pluripotency genes (Sox2, Pouf51, Nanog) and somatic genes (Setbp1, Col1a1, Col5a1). Data are presented as the mean ± S.D and n = 3 independent experiments. A two-tailed unpaired Student’s t test was used.