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. 2022 Dec 2;3:38. doi: 10.1186/s43556-022-00104-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Functional identification of eccDNAs in colorectal cancer cell lines. a Heatmap of differential expression of genes REBB2, MYCBPAP, TP53I3, EGFR, SLC7A1, SLC29A1 in the samples. b-c DNA electrophoresis of inverse PCR for genes in Fig. 5a to verify eccDNA in HCT116 and Lovo respectively. G: genome DNA. LE: total DNA with linear DNA elimination. RCA: total DNA with linear DNA elimination after RCA. d Schematic representation of the sequencing results of PCR products of gene SLC7A1 in samples after RCA with matching the sequence in chr13. e-f Relative mRNA expression and protein levels of SLC7A1 and β-actin in HCT116 transfected synthetic eccDNAs