Fig. 7.

Reanalysis of the human section of ProteomicsDB with the Picked Protein Group FDR method leads to increased information for databases including isoforms.A, bar plot showing the number of protein groups at 1% FDR. The Picked Protein Group FDR method exhibits an increase in the number of protein groups as isoforms and unreviewed proteins are included in the database. This is mainly because multiple protein groups can now be identified per gene. B, Venn diagram on gene level comparing the Savitski method without isoforms (red) and the Picked Protein Group FDR method with isoforms (blue). The Picked Protein Group FDR method reveals information about protein isoforms, with 8% of the identified genes having multiple identified protein groups (gray). FDR, false discovery rate.