Fig. 4.
The cytotoxic effect of ethanolic extract and alkaloid fraction of M. jalapa flowers. A total of 105 HepG2 cells were seeded in a 96-well microplate for 24 h and then incubated with 7 serial dilutions of the alkaloid fraction with MTT reagent from 3.9 to 250 ppm for 24 h. The following day, the 10 % SDS in 0.1 N HCl stop solution was added into each well of iron deficiency HepG2 cell model for overnight and the formazan formation in HepG2 cells was spectrophotometrically measured at 595 nm wavelength. The IC50 was determined by plotting the percentage of viable cells against AFF concentrations (ppm) using probit analysis. All experiments were performed in triplicate.