Fig. 7.

Results of MT-2 expression in DFO group and DFO treated with 100 µM FAC and 0.5, 1, or 2 IC₅₀ AFF groups. A total of 10⁵ HepG2 cells were grown in the coverslip of a 24-well microplate for 24 h, then induced with 100 µM DFO for 24 h (DFO group). The following day, the DFO group was treated with 100 µM FAC and 0.5, 1, or 2 IC₅₀ AFF. HepG2 normal cells, DFO group, and FAC/AFF treated-DFO groups were stained with monoclonal antibody anti-human MT-2 and secondary antibody conjugated streptavidin–horseradish peroxidase. All experiments were performed in duplicate. Red arrow: immunopositive; Blue arrow: immunonegative. The HepG2 normal cells provided the baseline data and were compared to the DFO group.