TABLE 5.
Detection of IL-5 in sera from intranasally immunized micea
Expt no. | Serum sample | IL-5 (U/ml) |
---|---|---|
1 | Control (naive) | NDb |
Immune | 3.88 | |
2 | Control | ND |
Immune | 4.44 |
Sera from mice intranasally immunized with 250 μg of TT plus 10 μg of CT (days 0 and 7) were assayed for IL-4, IL-5, and IFN-γ by ELISA. Significant levels of IL-5, but not IL-4 or IFN-γ, were detected. Sera from animals (n = 5) were pooled.
ND, not detected. The detection limit was 0.39 U/ml.