Table 1.
Quantification of PDK1 and CAMKK2 percent knockdown for single and combination knockdown in siRNA transfected cells
| Day | Individual siRNA |
Combination siRNA |
||
|---|---|---|---|---|
| PDK1 | CAMKK2 | PDK1 | CAMKK2 | |
| 5 | 63.6 ± 44.58 | 59.4 ± 19.5 | 56.1 ± 61.1 | 49.0 ± 54.6 |
| 7 | 83.9 ± 13.3 | 75.5 ± 17.8 | 88.5 ± 3.5 | 86.6 ± 12.3 |
| 9 | 85.1 ± 9.4 | 81.8 ± 9.0 | 93.2 ± 1.6 | 89.6 ± 5.2 |
OVCAR-3 cells were sequentially transfected with 40 μM NS or PDPK1 siRNA on Day 2 and 40 μM NS or CAMKK2 siRNA on Day 4. Values represent mean fold-change ± standard deviation relative to the NS siRNA. N=3 independent experiments. Obtained with permission from Gocher et al.1