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. 2022 Nov 30;3(4):101890. doi: 10.1016/j.xpro.2022.101890

Table 1.

Quantification of PDK1 and CAMKK2 percent knockdown for single and combination knockdown in siRNA transfected cells

Day Individual siRNA
Combination siRNA
PDK1 CAMKK2 PDK1 CAMKK2
5 63.6 ± 44.58 59.4 ± 19.5 56.1 ± 61.1 49.0 ± 54.6
7 83.9 ± 13.3 75.5 ± 17.8 88.5 ± 3.5 86.6 ± 12.3
9 85.1 ± 9.4 81.8 ± 9.0 93.2 ± 1.6 89.6 ± 5.2

OVCAR-3 cells were sequentially transfected with 40 μM NS or PDPK1 siRNA on Day 2 and 40 μM NS or CAMKK2 siRNA on Day 4. Values represent mean fold-change ± standard deviation relative to the NS siRNA. N=3 independent experiments. Obtained with permission from Gocher et al.1