TABLE 3.
Effect of oligopeptides derived from repeating units of Vsp antigens on the adherence of M. bovis to EBL cells
| Repeat(s) (sequence) | Oligopeptide(s) showing the following effect on the adherence rate (% change):
|
Adherence epitopeb | |
|---|---|---|---|
| Reductiona | No reduction | ||
| RA1 (PGENKT) | PGENKT (+6.9 ± 0.1) | TPGEN | |
| PGENK (+31.5 ± 3.2) | |||
| RA2 (PEENKK) | PEENKKPEENKK (−28.2 ± 4.4) | ENKKP (−2.0 ± 0.1) | KPEENK |
| NKKP (−5.0 ± 0.3) | |||
| KPEEN (+8.5 ± 1.3) | |||
| RA3 and RB1 (GTPANPDQ) | GTPANPDQ (+6.0 ± 0.3) | ||
| GTPANP (+9.2 ± 2.5) | |||
| RA4.1 and RB2.1 (GAGTKPGQ) | GAGTKPGQ (−26.2 ± 2.0) | GTK | |
| AGTKP (−20.1 ± 4.1) | |||
| AGTK (−33.2 ± 4.5) | |||
| GTKP (−33.4 ± 3.3) | |||
| RA4.2 and RB2.2 (GAGTNSQQ) | GAGTNSQQ (+6.8 ± 0.7) | ||
| AGTNS (−12.9 ± 1.9) | |||
| RE1 (PETPKG) | TPKGP (−24.4 ± 1.8) | PETPKG (+3.8 ± 0.5) | TPKGP |
| RF1 (see Fig. 4) | GNDLGKVQVAEQDK (−37.3 ± 5.0) | NDL | |
| KVPTLK (−21.0 ± 3.8) | TLK | ||
| KSVTV (−20.8 ± 3.3) | SKKS | ||
| KEKIETA (−0.6 ± 4.6) | KEK | ||
| KEAIVTK (−1.0 ± 4.4) | KEAIVTK | ||
| TLKFEI (+3.1 ± 0.2) | |||
| RF2 (QGTGAPKSPQ) | QGTGAPKSPQ (−25.0 ± 3.3) | GAPKS (−7.7 ± 1.8) | APKS |
a
Decrease in adherence rate of at least 20% in comparison to the value in the control trial (rate, 100).
b
The sequence was determined by epitope mapping on a membrane.