TABLE 4.
Verification of putative adherence sites with MAbs
| MAb | Recognized site(s) (repeat)a | Reduction of adherence rate (%)b |
|---|---|---|
| 1E5 | AGTK (RA4.1) | 34c |
| AGTN (RA4.2) | ||
| GNDLGKVQVA and SKKSVTV (RF1) | ||
| GTGA (RF2) | ||
| 4D7 | TPGENK (RA1) | 41 |
| KPEENK (RA2) | ||
| 2A8 | GENK (RA1) | 35 |
| NKKP (RA2) | ||
| (G)AGT (RA4.1) | ||
| SETLKS, KVPTL, and KSVTV (RF1) | ||
| 5D8d | 6c |
a
Amino acid residues belonging to adherence sites identified in this study are shown in bold type.
b
Standard conditions: 60 μg of affinity-purified MAb and 108 mycoplasma cells.
c
Data are from reference 21.
d
MAb 5D8 was included as a control. It was previously shown to recognize a nonvariant intracellular 41-kDa protein of M. bovis (19).