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. 2022 Dec 3;8:478. doi: 10.1038/s41420-022-01259-z

Fig. 6. Multiple critical molecules and signaling pathways were involved in Trastuzumab resistance.

Fig. 6

A The expressions of several membrane receptors and critical molecules of PI3K/AKT, MAPK, WNT, and cell cycle pathways were detected by western blot. β-actin as endogenous control; RTK, receptor tyrosine kinase. A-β-catenin, non-phospho β-catenin at Ser45. B The mRNA expressions of transcription factors c-Myc and TCF1/TCF7 were measured using quantitative PCR. *p < 0.05, **p < 0.01, ***p < 0.001. C The nuclear expressions of c-Myc, TCF1/TCF7 and β-catenin were evaluated by immunohistochemistry (IHC, x200 for the left row, x1000 for the right row). The staining score of IHC was defined as 0, 1+, 2+, and 3+.