Table 2.
PBD layout for AuNPs biosynthesis, using Bacillus subtilis NRC1 extracellular filtrate.
| Run | Casein hydrolysate | Dextrin | Initial pH | Incubation period | Inoculum size | Temperature | Maximum peak absorbance intensity | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coded | Actual (%) | Coded | Actual (%) | Coded | Actual | Coded | Actual (days) | coded | Actual (CFU × 106/ml) | coded | Actual (°C) | Experimental value | Predicted value | |
| 1 | 0 | 0.44 | 0 | 2.5 | 0 | 7 | 0 | 3 | 0 | 168 | 0 | 30 | 2.78 | 2.88 |
| 2 | 1 | 0.72 | −1 | 1 | 1 | 8 | 1 | 5 | −1 | 56 | 1 | 40 | 0.74 | 0.77 |
| 3 | −1 | 0.16 | 1 | 4 | −1 | 6 | 1 | 5 | 1 | 280 | −1 | 20 | 0.19 | 0.19 |
| 4 | 0 | 0.44 | 0 | 2.5 | 0 | 7 | 0 | 3 | 0 | 168 | 0 | 30 | 3 | 2.88 |
| 5 | −1 | 0.16 | 1 | 4 | 1 | 8 | −1 | 1 | 1 | 280 | 1 | 40 | 1.8 | 1.80 |
| 6 | 1 | 0.72 | 1 | 4 | −1 | 6 | −1 | 1 | −1 | 56 | 1 | 40 | 0.91 | 0.88 |
| 7 | −1 | 0.16 | −1 | 1 | −1 | 6 | −1 | 1 | −1 | 56 | −1 | 20 | 0 | 0.03 |
| 8 | 1 | 0.72 | 1 | 4 | −1 | 6 | 1 | 5 | 1 | 280 | 1 | 40 | 1.14 | 1.17 |
| 9 | −1 | 0.16 | −1 | 1 | −1 | 6 | 1 | 5 | −1 | 56 | 1 | 40 | 0.53 | 0.50 |
| 10 | −1 | 0.16 | −1 | 1 | 1 | 8 | −1 | 1 | 1 | 280 | 1 | 40 | 1.09 | 1.09 |
| 11 | −1 | 0.16 | 1 | 4 | 1 | 8 | 1 | 5 | −1 | 56 | −1 | 20 | 2.73 | 2.73 |
| 12 | 1 | 0.72 | −1 | 1 | −1 | 6 | −1 | 1 | 1 | 280 | −1 | 20 | 1.53 | 1.53 |
| 13 | 0 | 0.44 | 0 | 2.5 | 0 | 7 | 0 | 3 | 0 | 168 | 0 | 30 | 2.87 | 2.88 |
| 14 | 1 | 0.72 | 1 | 4 | 1 | 8 | −1 | 1 | −1 | 56 | −1 | 20 | 2.03 | 2.03 |
| 15 | 1 | 0.72 | −1 | 1 | 1 | 8 | 1 | 5 | 1 | 280 | −1 | 20 | 0.64 | 0.61 |