Fig. 8.

TREM2 depletion abolished the effects of COG1410 on short-term neurobehaviour and the Akt/CREB/BDNF signalling pathway. A–C Quantitative analysis of short-term neurological function by NSS scores (A), wire grip scores (B), and rotarod test (C) at 3 d after CCI. *p < 0.05 vs. WT CCI + COG1410; ^#p < 0.05 vs. WT CCI + Vehicle; ns p > 0.05 KO CCI + Vehicle vs. KO CCI + COG1410, n = 6 per group. D Representative Western blot bands of TREM2, p-Akt, Akt, p-CREB, CREB, BDNF, TNF-α, IL-1β, cleaved-caspase-3, and β-Actin at the lesion sites after CCI. E–K Quantitative analysis of TREM2 (E), p-Akt (F), p-CREB (G), BDNF (H), TNF-α (I), IL-1β (J), and cleaved-caspase-3 (K) density at the lesion sites after CCI. *p < 0.05 vs. WT CCI + COG1410; ^#p < 0.05 vs. WT CCI + Vehicle; ns p > 0.05 KO CCI + Vehicle vs. KO CCI + COG1410, n = 6 per group