TABLE 1.
List of strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| Porphyromonas gingivalis | ||
| W83 | Laboratory stock | |
| 4351 | W83 Δdpp | Present study |
| 4361 | W83 Δdpp | Present study |
| EM23 | W83 dpp::φ (ermF-ermAM) | Present study |
| Escherichia coli | ||
| SY327λpir | Δ(lac pro) argE(Am) rif nalA recA56 pir | 24 |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km pir | 24 |
| BL21 | hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene1) | 36 |
| DH1 | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 36 |
| DPPRWT | BL21/pYKP406 | Present study |
| DPPRSA | BL21/pYKP407 | Present study |
| DPPRDA | BL21/pYKP408 | Present study |
| DPPRHA | BL21/pYKP409 | Present study |
| Plasmids | ||
| pUC119 | Apr | 36 |
| pUC4K | Apr Kmr | 42 |
| pGP704 | oriR6K mobRP4 Apr | 24 |
| pTD-T7 | Apr T7 promoter | 8 |
| pYKP001 | pVA2198 Kmr | Present study |
| pYKP002 | pGP704 φ(ermF-ermAM) Aps | Present study |
| pYKP009 | pGP704 φ(ermF-ermAM) Aps; one SphI site was eliminated | Present study |
| pYKP200 | pYKP009 ALAR fragment | Present study |
| pYKP300 | pBR322 dpp | Present study |
| pYKP301 | pYKP300 dpp::φ (ermF-ermAM) | Present study |
| pYKP403 | pTD-T7 dpp coding for DPPIV with a putative signal sequence | Present study |
| pYKP406 | pTD-T7 dpp coding for DPPIV without a putative signal sequence | Present study |
| pYKP407 | pTD-T7 dpp expressing S593A | Present study |
| pYKP408 | pTD-T7 dpp expressing D668A | Present study |
| pYKP409 | pTD-T7 dpp expressing H700A | Present study |
a
Apr, ampicillin resistant; Aps, ampicillin sensitive; Kmr, kanamycin resistant, oriR6K, replication origin of plasmid R6K; mobRP4, oriT region of plasmid RP4.