Fig. 3. TAK-981 activates dendritic cells ex vivo.
(A) Cytokines were measured in cell culture medium from human DCs, isolated from the peripheral blood of two healthy donors, following 16h of treatment with DMSO or the indicated concentrations of TAK-981 (n = 10 for each treatment). Horizontal lines represent mean and SD. *P < 0.05, **P < 0.01, ****P < 0.0001, compared with DMSO control, by Welch’s t test. (B) The percent of CD40, CD80 and CD86 positive DCs was assessed by flow cytometry of human DCs isolated from the peripheral blood of two healthy donors following 16h of ex vivo treatment with DMSO or the indicated concentrations of TAK-981 (n = 10 for each treatment). Horizontal lines represent mean and SD. ****P < 0.0001, compared with DMSO control, by Welch’s t test.