TABLE 2.
Antigen components in S. sobrinus ultrasonic fragments after proteinase digestion and hydrolysis by acetic acid
| Treatment | Treatment time (min) | Protein concn (μg/ml) | Saccharide concn (μg/ml) | Antibody reactivitya (μg/ml) |
|---|---|---|---|---|
| None | 0 | 137.5 | 57.2 | 30.7 |
| Elimination after heat denaturation | 10 | 12.5 | 52.3 | 25.5 |
| Trypsin digestion | 60 | 13.8 | 55.7 | 26.1 |
| Hydrolysis by acetic acid | 30 | 9.7 | 44.6 | 20.4* |
| 60 | 9.3 | 23.1 | 10.2* | |
| 90 | 9.3 | 3.2 | 1.7* |
a
Antibody concentrations were expressed as means ± SD, and the significance was determined using Student's t test. ∗, P < 0.01 versus nontreatment.