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. 2022 Nov 15;66:101637. doi: 10.1016/j.molmet.2022.101637

Figure 3.

Figure 3

RvD1, but not RvE1, stimulates mitochondrial metabolism in human macrophages. (A) Human PBMC were subjected to flow cytometric analysis to quantify expression level of monocyte-macrophage cell surface markers. Unstained samples shown in grey histograms while stained ones are shown in respective colored histograms: GM–CSF–differentiated hMDM in purple (top row) while M–CSF–differentiated hMDM are shown in green (bottom row). (B) Quantification of CD14, CD16, CD64, and CD206 MFI following differentiation in GM-CSF and M-CSF. Grey dashed line shows the MFI for the respective marker on undifferentiated hPBMC. (C, E) Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) values following treatment without or with 1 nM RvD1 or RvE1 in GM-CSF or M-CSF differentiated MDM. (D, F) Quantification of derived mitochondrial respiratory parameters. Data expressed as mean ± SEM; n = 3 for all groups; ∗∗P < 0.01; One-way ANOVA with Holm-Šídák post-test.